I want to investigate how different disinfectant concentrations can affect the reproduction and growth
of micro-organisms, bacteria.
Hypothesis:
My hypothesis is, the more concentrated the dettol the more bacteria it will kill.
clear zone.
The variable that I will be changing is the concentration of the disinfectant I will do this by adding the
same amount of disinfectant to different amounts of water to change its strength.
I will use:
- Full strength dettol
- Manufacturers instructions
- No dettol - just water
- Diluted dettol
The variable that I will measure is the size of the clear zone around the Dettol disk.
- Growing temperature - we need to grow bacteria in the same temperature for each.
- Same environment - We need to grow the bacteria on the same medium (Seaweed jelly)
- Growing time - We need to grow the bacteria for the same amount of time.
- Size of the disc - we need to make sure we have the same amount of dettol .
- The age of the dettol, the length of time the seal has been broken i.e. shelf life
Gather the equipment
- Dettol
- Vivid - for labeling
- Filter paper
- Water
- Salatape
- Agar plate
- Hole punch
- Bacterial solution, a solution of bacteria
- Pipette
- Tweezers
Get the filter paper and cut out 4 disks with the hole punch.
Step 3
Using your vivid, mark out the agar plate into four quadrants (sections), label each quadrant so you can identify which section is which, do not label the lid, label the bottom of the agar plate.
Swirl the bacteria around in the agar plate, with the lid on, to cover all the surface.
Tip out the excess bacteria solution in the agar plate into a sink, do NOT rinse agar plate.
place it into the appropriate quarter.
Using the tweezers, pick up the second disc and dip them into the dettol, shake off any excess drops and place it into the appropriate quarter.
Make up the next solution in a measuring cylinder, put 400 ml of water in the cylinder and one cap full of dettol. Mix together and pick up the third disc, dip them into the watery dettol, shake off excess drops and place into the appropriate agar plate quadrant
Make up the last solution in a measuring cylinder, put 800 ml of water into the cylinder and one cap full of dettol. Swirl to mix together and pick up the third disc, dip them into the watery dettol, shake off excess drops and place into the agar plate quadrant
Incubate at 20 degrees, upside down. Leave it over the weekend and come back tuesday!
Repeat 2 more times so you have 3 results.
During our test, bacteria was able to grow in the environment given. But due to the dettol, bacteria was killed off, the clear zone increased with the larger amounts of concentrated dettol added.
During the process, dettol seeps into the bacteria and breaks down the cell wall, seeping right into the cytoplasm. The chemical in dettol, Chloroxylenol, stops the bacteria from producing ATP (produced by proton gradient distributor), Adenosine TriPhosphate or ATriPhosphate. ATP is the energy storer, it responds to the bodies need for energy, i.e. moving, breaking, or eating, this stops the bacteria from reproducing and gathering nutrition, promptly starving the bacteria to death. The ATP’s are produced by proton gradient distributor, by shutting this down ATP can not be produced.
The enzymes within bacteria gets shut down, the particular enzymes that are able to degrade down nutrients, organic materials, such as fats, oils, cellulose (an organic compound with multiple linked glucose units), xylan (found within plant cell walls and some algaes), proteins, and starches, are shut down due to the chloroxylenol within dettol. This will also stop the bacteria from being able to gather nutrients, again, promptly starving the bacteria to death.
Chloroxylenol is not toxic to humans, non toxic to birds and is moderately toxic to freshwater invertebrates (Animals that do not possess vertebrate columns (Spine)). The chemical is known to be dangerous to cats, and is extremely lethal to the cane toad. The chemical was banned from being used as pest control in western Australia due to concerns of flora and fauna potentially being harmed.
The cell wall is what bacteria uses as protection, it being a key part of its survival. It provides the
bacteria structure, it also stops water from rushing into the bacteria and disrupting the large amounts
of protein they contain. The thickness of a cell wall can help identify weather the bacteria is gram
positive (Thicker cell wall, dettol being more effective on this type) or gram negative (Thinner cell wall,
dettol being less effective on this type), identification of this is called Gram Staining.
Although this effective, bacteria cell walls have holes in it, these holes are what allowed dettol to enter
the bacteria's system.
various diseases, such as salmonella, this is done by enhancing the ability to create and spread the
diseases. The capsule is found in both gram negative and gram positive bacteria.
various diseases, such as salmonella. The capsule is found in both gram negative and gram positive
bacteria.
enclosed within membranes)
sugar molecules bonded together. The background, surface, that the bacteria resides on is stained
darker than the bacteria. The capsule is the light, either pale or colorless, residing as the outer layer
like a halo or ring.
bacterium, has this layer. In this bacteria, the cell wall resides underneath capsule, the outer layer.
The cell wall is what bacteria uses as protection, it being a key part of its survival. It provides the
bacteria structure, it also stops water from rushing into the bacteria and disrupting the large amounts
of protein they contain. The thickness of a cell wall can help identify weather the bacteria is gram
positive (Thicker cell wall, dettol being more effective on this type) or gram negative (Thinner cell wall,
dettol being less effective on this type), identification of this is called Gram Staining.
Although this is effective, bacteria cell walls have holes in it, these holes are what allowed dettol to
enter the bateria’s system.
maintaining the cell structure, holding proteins and linking together tissues and cellular components.
The cellular components provide hydrophilic signals, a molecule that has a better reaction with water
and other polar substances, than the reaction with oil or other solvents. The membrane contains
proteins which transport protein makers and salt in and out, which help other proteins attach and act
as a identifier for the bacteria. This helps the membrane communicate with each other.
jelly like fluid.
and aiding in metabolic activity (The life sustaining chemical transformation within cells)
bacteria have a more solid form.
formation of specific proteins, they are the cellular component that makes proteins from amino acids.
Ribosomes are made up of RNA, ribonucleic acid, RNA is a molecule essential in coding, decoding,
regulation and expression of genes. RNA is almost identical to DNA, but RNA is made of ribose sugar
instead of deoxyribose sugar and have nucleotides with a uracil base instead of thymine.
this can be replicated independently. When a bacteria is under stress, the plasmid helps keep them
alive longer, this gives the bacteria a better opportunity to reproduce and pass the plasmid onto their
daughter cells.
by plasmid provide bacteria with genetic advantages, such as a resistance to antibiotics.
cell surface. The pili are shorter, straighter and more numerous than the flagella and are composed by
subunits of protein.
attachment between bacteria.
micrometers in diameters, averaging to around 2 micrometers.
Peritrichous.
structure.
to the cell wall.
occurring in a variety of organisms, such as bacteria.
First it replicates its DNA, elongates, splits and produces two equal daughter cells.
occurs because of mutations present during binary fission.
impossible due to bacteria taking random times for each step, approximately adding up to around
20 minutes.
operated by proteins, more specifically transport proteins, these proteins reside within the plasma
membrane, these proteins and plasma membrane act as a sponge, taking in nutrients in large numbers
to keep the bacteria energized and healthy.
transported through the membrane by the transport systems, the transport systems have three ways to
transport the nutrients through.
line.
the membrane in the same direction.
solute is transported into the bacteria, while the other is transported out, simultaneously.
The solutes consist of carbons, such as sugars, fats, and amino acids. The plasma membrane lets these solutes in, but the enzymes are what breaks down the complex compounds to use the nutrients.
Plate 1
|
Plate 2
|
Plate 3
|
Plate 4
|
Average
| ||
Full Strength
|
30 mm
|
25 mm
|
30 mm
|
30 mm
|
31.67
| |
Manufactures Instructions
|
25 mm
|
10 mm
|
30 mm
|
30 mm
|
18.33
| |
Diluted
|
0 mm
|
10 mm
|
25 mm
|
25 mm
|
10.32
| |
Water
|
0 mm
|
0 mm
|
0 mm
|
0 mm
|
0
| |
Plate 4 is an unbelievable results. Full strength dettol would kill more
than the manufacturer's instructions. I am ruling this plate out as untrue. The reasons it could have been messed up is because of the tweezers used, the examiners mostlikely didn't rinse the tweezers before taking a sample of the manufacturers instructions and therefore left the disk with pure dettol on it. This can cause more bacteria to die and result in a larger clear zone, as seen in the results. |
But, if I were to use undiluted dettol on a tile floor it would be a waste of dettol, the moment dettol
seeps into the bacterias system the bacteria is set to die, using the diluted dettol will kill off bacteria
effectively and will not waste any dettol.
odd result, this result was left out of the graph. All instructions were followed correctly to get the best
results in the experiment giving a more accurate result. Our results were off due to the agar plate not
being placed in the incubation chamber straight away, this proved difficult for my results but due to
having other results to work with I could work out the information needed.
calculate my results.
accurately when killed off. This provided good information to help calculate the information needed for
my results.
Here are the other results I used
and fire-fighting foam. This chemical is a Heteroatoms, a compound of 3 chemicals.
includes amino acids, the acid that creates protein, usually occurring within blood and tissue.
Note -
I worked with Kimberly during this experiment
http://hhskimberleym.blogspot.com/



